Quantitative Chromatographic Separations of Synthetic Peptides

Chromatographic Studies of Protein-Based Chiral Separations

The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used a...

Chromatographic Separations with Selected Supported Chelating Agents

Isoelectric Point Separations of Peptides and Proteins

The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also dis...

Development of flexible and efficient strategies for optimizing chromatographic separations.

The intensive research in chemometrics is resulting in continuous development of new concepts and optimization methods. The practical chromatographic optimization examples described in this paper highlight the importance of developing efficient and flexible optimization strategies, which are adapted to the (complex) separation problems encountered in the real chromatographic world. The availabi...

Use of Simulated Annealing for Optimization of Chromatographic Separations

The effectiveness of a hybrid of simulated annealing (SA) and the simplex algorithm for optimization of a chromatographic separation has been examined. The method has been used to solve optimization problems for separations in batch and continuous chromatographic systems under isocratic and gradient conditions. To verify the robustness of the algorithm a variety of test problems have been solve...